-
-
유전자 삽입 장치
- 보유부서비임상지원센터 오송첨단의료산업진흥재단
- 제조사Nepagene
- 모델명NEPA21
장비개요
-In Vitro Transfection into difficult-to-transfect cells such as primary cells stem cells etc. into any cell lines with electroporation cuvettes with cell-culture-plate electrodes for cells in adherence -In Vivo Transfection into mouse/rat brain eye muscle skin liver kidney testis etc. with in vivo electrodes -In Utero Transfection into mouse/rat embryos: cerebral cortex hippocampus spinal cord etc. with in utero electrodes -In Ovo Transfection into chick embryos: neural tube limb bud digestive organ etc. with in ovo electrodes -Ex-Vivo Transfection into explants brain slices organs whole embryos etc. with ex vivo electrodes
장비상세정보
| 취득일자 | 2013-12-03 | 제작국가 | 일본 |
|---|---|---|---|
| 장비상태 | 활용 | 활용범위 | 기관외부활용 |
| 장비용도 | 분석 | 설치장소 | 충청북도 청주시 흥덕구 오송읍 오송생명로 123 오송첨단의료산업진흥재단 비임상지원센터 F1층 1204호 |
| 연락처 | 0432009862 | ||
규격 및 특징
1. Poring pulse mode
1) In Vivo In Utero In Ovo Ex vivo 모드
(1) Voltage : 0.1 ～ 300 V (0.1 V씩 증가)
(2) Pulse length : 0.1 ～ 99.9 ms (0.1 ms씩 증가)
(3) Pulse interval : 10 ～ 99.9 ms (0.1 ms씩 증가)
(4) Number of pulse : 0 ～ 9
(5) Decay rate : 0 ～ 99%
2) In vitro Adherence cell 모드
(1) Voltage : 0.1 ～ 300V (0.1 V씩 증가)
(2) Pulse length : 250.1 ～ 300.0V : 0.1 ～ 15.0 ms (0.1 ms씩 증가) 200.1 ～ 250.0V : 0.1 ～ 25.0 ms (0.1 ms씩 증가) 150.1 ～ 200.0V : 0.1 ～ 50.0 ms (0.1 ms씩 증가) 0.1 ～ 150.0V : 0.1 ～ 99.9 ms (0.1 ms씩 증가)
(3) Pulse interval : 50 ～ 99.9 ms (0.1 ms씩 증가)
(4) Number of pulse : 0 ～ 9
(5) Decay Rate : 0 ～ 99%
2. Transfer pulse 모드
1) Voltage : 0.1 ～ 99.9 V (0.1V씩 증가)
2) Pulse length : 0.1 ～ 99.9 ms (0.1 ms씩 증가)
3) Pulse interval : 10.0 ～ 999.9 ms (0.1 ms씩 증가)
4) Number of pulses : 0 ～ 99
5) Decay rate : 0 ～ 99 %
6) Polarity switching (1) on: with polarity switching (2) off: without polarity switching
3. Output measurements
1) Voltage
(1) poring pulse : 0.0 ～ 350.0 V
(2) transfer pulse : 0.0 ～ 150.0 V
2) Current
(1) Poring pulse : 0.000 ～ 9.999 A (0.001 A씩 증가) 10.00 ～ 99.99 A (0.01 A씩 증가)
(2) Transfer pulse : 0.000 ～ 9.999 A (0.001 A씩 증가) 10.00 ～ 99.99 A (0.01 A씩 증가)
3) Energy
(1) Poring pulse : 0.000 ～ 9.999 J (0.001 J씩 증가) 10.00 ～ 99.99 J (0.01 J씩 증가) 100.0 ～ 999.9 J (0.1 J씩 증가)
(2) Transfer pulse : 0.000 ～ 9.999 J (0.001 J씩 증가) 10.00 ～ 99.99 J (0.01 J씩 증가) 100.0 ～ 999.9 J (0.1 J씩 증가)